Journal: Antiviral Chemistry & Chemotherapy

Article Title: Prodrugs of herpes simplex thymidine kinase inhibitors

doi: 10.1177/2040206615608722

Figure Lengend Snippet: Aqueous solubilities of prodrugs (mg/ml). a

Article Snippet: However, solubilities increased dramatically in the presence of various adjuvants such as Soluplus®, Solutol®, and Kollidon®, registered trademarks of BASF.

Techniques:

Journal: Antiviral Chemistry & Chemotherapy

Article Title: Prodrugs of herpes simplex thymidine kinase inhibitors

doi: 10.1177/2040206615608722

Figure Lengend Snippet: Plasma concentration of m CF 3 PG after bolus gavage dosing of sacrovir to mice. a

Article Snippet: However, solubilities increased dramatically in the presence of various adjuvants such as Soluplus®, Solutol®, and Kollidon®, registered trademarks of BASF.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Antiviral Chemistry & Chemotherapy

Article Title: Prodrugs of herpes simplex thymidine kinase inhibitors

doi: 10.1177/2040206615608722

Figure Lengend Snippet: Consumption of sacrovir in drinking water by mice. a

Article Snippet: However, solubilities increased dramatically in the presence of various adjuvants such as Soluplus®, Solutol®, and Kollidon®, registered trademarks of BASF.

Techniques: Clinical Proteomics

Journal: Antiviral Chemistry & Chemotherapy

Article Title: Prodrugs of herpes simplex thymidine kinase inhibitors

doi: 10.1177/2040206615608722

Figure Lengend Snippet: Effect of treatments on HSV-1 reactivation in mice.

Article Snippet: However, solubilities increased dramatically in the presence of various adjuvants such as Soluplus®, Solutol®, and Kollidon®, registered trademarks of BASF.

Techniques: Control, Positive Control

Journal: International Journal of Nanomedicine

Article Title: Optimization and Pharmacokinetic Evaluation of Synergistic Fenbendazole and Rapamycin Co-Encapsulated in Methoxy Poly(Ethylene Glycol)- b -Poly(Caprolactone) Polymeric Micelles

doi: 10.2147/IJN.S315782

Figure Lengend Snippet: Characteristics of Fenbendazole (FEN) and Rapamycin (RAPA) Encapsulated in Various Polymers (n = 3)

Article Snippet: Soluplus ® [Polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (PCL-PVAc-PEG)] was obtained from BASF (Ludwigshafen, Rhineland-Palatinate, Germany).

Techniques: Polymer, Encapsulation, Zeta Potential Analyzer

Journal: Diagnostics

Article Title: Hypotensive Effect of Nanomicellar Formulation of Melatonin and Agomelatine in a Rat Model: Significance for Glaucoma Therapy

doi: 10.3390/diagnostics10030138

Figure Lengend Snippet: Lowering effect of melatonin formulated at 0.4% in saline or in nanomicelles in rats with normotensive intraocular pressure. No difference in the magnitude of lowering effect could be assessed among the different formulations. The nanomicellar formulation increased the duration of the hypotonizing effect. Values represent the mean ± standard error of the mean (SEM) of data from 3 rats (6 eyes).

Article Snippet: The formulation of melatonin and agomelatine (Molekula) in nanomicelles (Soluplus ® , 115.000 g/mol; BASF, Ludwigshafen, Germany) was prepared by the direct dissolution method.

Techniques: Saline, Formulation

Journal: Diagnostics

Article Title: Hypotensive Effect of Nanomicellar Formulation of Melatonin and Agomelatine in a Rat Model: Significance for Glaucoma Therapy

doi: 10.3390/diagnostics10030138

Figure Lengend Snippet: Statistical analysis of data shown in Figure 2 . Differences were calculated with respect to the intraocular pressure measured in rats instilled with saline.

Article Snippet: The formulation of melatonin and agomelatine (Molekula) in nanomicelles (Soluplus ® , 115.000 g/mol; BASF, Ludwigshafen, Germany) was prepared by the direct dissolution method.

Techniques: Saline

Journal: Diagnostics

Article Title: Hypotensive Effect of Nanomicellar Formulation of Melatonin and Agomelatine in a Rat Model: Significance for Glaucoma Therapy

doi: 10.3390/diagnostics10030138

Figure Lengend Snippet: Lowering effect of melatonin and agomelatine at 0.4% without or with lipoic acid formulated in nanomicelles in rats with hypertensive intraocular pressure. No difference in the magnitude of lowering effect could be assessed after the addition of lipoic acid that, however, increased the duration of the mixture efficacy. Data represent the mean ± SEM of data from 3 rats (6 eyes).

Article Snippet: The formulation of melatonin and agomelatine (Molekula) in nanomicelles (Soluplus ® , 115.000 g/mol; BASF, Ludwigshafen, Germany) was prepared by the direct dissolution method.

Techniques:

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: (a) Schematic illustration of self-assembled Soluplus-BA micelles. (b) Micelle size. (c) Transmission electron microscopy (TEM) images of Soluplus-BA (magnification ×400 k, bar =50 nm).

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Transmission Assay, Electron Microscopy

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles inhibit proliferation of breast cancer MDA-MB-231 cells. (A) MDA-MB-231 cells were treated with Soluplus, BA, or Soluplus-BA micelles for 48 h, cell viability was detected by MTT assay. (B) MDA-MB-231 cells (1000 cells/well) were seeded in 6-well plates and treated with BA or Soluplus-BA for 48 h, after changing to fresh culture medium, and continued to culture for 10 days, the cells were stained with crystal violet. ** p < 0.05 vs. control group, ## p < 0.05 vs. BA group.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: MTT Assay, Staining

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Cell uptake assay. MDA-MB-231 cells were treated with free cou6 and Soluplus-cou6 micelles for 3 min, 5 min, and 15 min, fluorescence was observed and photographed with a fluorescence microscope (Bar = 50 µm).

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Fluorescence, Microscopy

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Intracellular trafficking. MDA-MB-231 cells were stained with Lyso-Tracker Red or Mito-Tracker Red for 20 min, after 3-times of washes with PBS, cells were treated with Soluplus-cou6 micelles for 5 min. (A) Colocalization micrographs of micelles with lysosomes. (B) Colocalization micrographs of micelles with mitochondria (Bar = 20 µm).

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Staining

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles induce ROS-mediated mitochondrial apoptosis pathway. MDA-MB-231 cells were treated with Soluplus-BA or BA for 48 h. Cells were stained with DCFH-DA (20 min) or JC-1 (30 min) and photographed under a fluorescence microscope (Bar = 50 µm).

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Staining, Fluorescence, Microscopy

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles induce DNA double-strand breaks. (A) MDA-MB-231 cells were treated with BA or Soluplus-BA micelles for 48 h, the expression of γH2AX was detected by immunofluorescence. (Bar = 50 µm). (B) Expression of γH2AX was analyzed by Western blot. ** p < 0.05 vs control group, ## p < 0.05 vs. free BA group.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles inhibit tube formation and ex vivo angiogenesis of CAM. (A) HUVEC cells were seeded in into the Matrigel-coated 96-well plates with Soluplus-BA or BA. After 6 h of incubation, the tube-like networks were photographed (×100). (B) Fertile chicken eggs are placed in the incubator and incubated for 6 days, a small opening was made at the top of eggs and 200 µl of Soluplus-BA micelles or BA was added and incubated for 24 h and photographed. (C) Effect of Soluplus-BA on the expression of HIF-1, VEGF and FAK. ** p < 0.05 vs. control group, ## p < 0.05 vs. free BA group.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Ex Vivo, Incubation, Expressing

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles inhibit migration. MDA-MB-231 cells were seeded in 24-well plates and incubated at 37 °C. After the cells grew to 90% confluence, they were scratched with a 200 µl pipette tip. The cells were treated with Soluplus-BA or BA, and photographed at 0, 12, and 24 h, respectively.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Migration, Incubation, Transferring

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Soluplus-BA micelles inhibit tumor growth in vivo. (A) 4T1 cells were inoculated into the armpits of mice, and the mice were orally administered with PBS, Soluplus, free BA (50 mg/kg), Soluplus-BA (25 mg/kg) and Soluplus-BA (50 mg/kg). Tumor volume was recorded every three days. (B) Body weight. (C) Immunohistochemistry analysis of the expression of Ki67, CD31 and γH2AX (Bar = 200 µm). S-BA: Soluplus-BA micelles. ** p < 0.05 vs. control group, ## p < 0.05 vs. free BA group.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: In Vivo, Immunohistochemistry, Expressing

Journal: Drug Delivery

Article Title: Improved anticancer activity of betulinic acid on breast cancer through a grafted copolymer-based micelles system

doi: 10.1080/10717544.2021.1979125

Figure Lengend Snippet: Hematoxylin-eosin (H&E) staining analysis morphological of visceral tissues (Bar = 200 µm). S-BA: Soluplus-BA micelles.

Article Snippet: PVCL-PVA-PEG (Soluplus) was purchased from BASF Ltd. (Shanghai, China).

Techniques: Staining